The Light Cycler 480 Probes Master is designed for real-time PCR using the Light Cycler 480 Instrument in combination with suitable probes (e.g., hydrolysis probes, Universal Probe Library probes, and others) and gene-specific primers. The kit is ideally suited for hot start PCR assays for gene quantification.

LightCycler® 480 Probes Master is a ready-to-use reaction mix specifically developed for the hydrolysis probe detection format in multiwell plates on the LightCycler® 480 Instrument. It contains FastStart Taq DNA Polymerase for hot start PCR, which significantly improves the specificity and sensitivity of PCR by minimizing the formation of nonspecific amplification products.

Benefits

1. Save time with a convenient, ready-to-use 2x-concentrated hot start master mix.
   
2. Minimize pipetting steps and contamination risk.
   
3. Perform sensitive, specific, and quantitative PCR or RT-PCR using the LightCycler® 480 Instruments.
   
4. Eliminate time-consuming MgCl2 titration.
   
5. Achieve consistent, high-quality performance with the LightCycler® 480 Instrument and Multiwell Plates.
   
6. Establish new qPCR assays and get results quickly by combining LightCycler® 480 Probes Master with Universal ProbeLibrary probes.